Mutations in EBF3 disturb transcriptional profiles and underlie a novel syndrome of intellectual disability, ataxia and facial dysmorphism

نویسندگان

  • Frederike Leonie Harms
  • Katta Mohan Girisha
  • Andrew A. Hardigan
  • Anju Shukla
  • Malik Alawi
  • Ashwin Dalal
  • Lauren Brady
  • Lynne M. Bird
  • Sophia Ceulemans
  • Martina Bebin
  • Susan M. Hiatt
  • Edward J. Lose
  • Michelle Primiano
  • Wendy K. Chung
  • Jane Juusola
  • Zeynep C. Akdemir
  • Matthew Bainbridge
  • Wu-Lin Charng
  • Mohammad K. Eldomery
  • Ayman W. El-Hattab
  • M. Saleh
  • Stéphane Bézieau
  • Benjamin Cogné
  • Bertrand Isidor
  • Sébastien Küry
  • James R. Lupski
  • Richard M. Myers
  • Gregory M. Cooper
  • Kerstin Kutsche
چکیده

76 From a GeneMatcher-enabled international collaboration, we identified ten individuals with 77 intellectual disability, speech delay, ataxia and facial dysmorphism and a mutation in EBF3, 78 encoding a transcription factor required for neuronal differentiation. Structural assessments, 79 transactivation assays, in situ fractionation, RNA-seq and ChIP-seq experiments collectively 80 show that the mutations are deleterious and impair EBF3 transcriptional regulation. These 81 findings demonstrate that EBF3-mediated dysregulation of gene expression has profound 82 effects on neuronal development in humans. 83

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تاریخ انتشار 2016